{"id":577,"date":"2023-02-08T09:52:48","date_gmt":"2023-02-08T00:52:48","guid":{"rendered":"https:\/\/home.hirosaki-u.ac.jp\/crobrdlab\/?page_id=577"},"modified":"2023-02-08T09:52:48","modified_gmt":"2023-02-08T00:52:48","slug":"dna%e7%a0%94%e7%a9%b6%e3%81%ab%e3%81%a4%e3%81%84%e3%81%a6%ef%bc%88%e5%9f%ba%e7%a4%8e%e7%b7%a8%ef%bc%89","status":"publish","type":"page","link":"https:\/\/home.hirosaki-u.ac.jp\/crobrdlab\/dna%e7%a0%94%e7%a9%b6%e3%81%ab%e3%81%a4%e3%81%84%e3%81%a6%ef%bc%88%e5%9f%ba%e7%a4%8e%e7%b7%a8%ef%bc%89\/","title":{"rendered":"DNA\u7814\u7a76\u306b\u3064\u3044\u3066\uff08\u57fa\u790e\u7de8\uff09"},"content":{"rendered":"

DNA\u306e\u591a\u69d8\u6027\uff1a<\/p>\n

\u591a\u578b\uff08\u305f\u3051\u3044\uff09\u3068\u3082\u3044\u3046DNA\u306e\u9055\u3044\u304c\u307f\u3089\u308c\u308b\u4f55\u304c\u308f\u304b\u308b\u306e\u3060\u308d\u3046\u304b\uff0e\u30a4\u30cd\u306e\u5728\u6765\u7a2e\u306e\u9055\u3044\uff0c\u54c1\u7a2e\u8b58\u5225\uff0cDNA\u306a\u3089\u30b3\u30e1\u3082\u500b\u4f53\u306e\u8b58\u5225\u304c\u3067\u304d\u307e\u3059\uff0e\u305d\u306e\u305f\u3081\u306bDNA\u62bd\u51fa\u3068\u591a\u578b\u306e\u6bd4\u8f03\u304c\u5fc5\u8981\u3067\u3042\u308a\uff0c\u305d\u306e\u65b9\u6cd5\u306b\u306f\u751f\u7269\u7a2e\u306b\u3088\u3063\u3066\u4f7f\u3048\u308b\u624b\u6bb5\u304c\u9650\u3089\u308c\u3066\u304d\u307e\u3057\u305f\uff0e\u3057\u304b\u3057\uff0c\u73fe\u5728\u306f\u3042\u3068\u3067\u89e6\u308c\u308b\u6b21\u4e16\u4ee3\u30b7\u30fc\u30af\u30a8\u30f3\u30b5\u30fc\u3084MIG-seq\uff08Suyama and Matsuki 2015\uff1aScientific Reports 5:16963\uff09\u3068\u547c\u3070\u308c\u308b\u65b9\u6cd5\u304c\u3042\u308b\u305f\u3081\uff0c\u30b3\u30b9\u30c8\u3068\u306e\u517c\u306d\u5408\u3044\u3067\u9078\u629e\u3067\u304d\u308b\u65b9\u6cd5\u304c\u5897\u3048\u3066\u304d\u307e\u3057\u305f\uff0e<\/p>\n

\u30a4\u30cd\u3067\u306f\u4e00\u5869\u57fa\u591a\u578b\uff08\u3053\u308c\u306f\u30b3\u30b9\u30c8\u9ad8\u304f\u306a\u308a\uff0c\u5fc5\u8981\u306a\u8abf\u67fb\u6a5f\u6750\u304c\u591a\u304f\u306a\u308a\u307e\u3059\uff09\uff0c<\/p>\n

RAPD\uff08\u30e9\u30f3\u30c0\u30e0\u30d7\u30e9\u30a4\u30de\u30fc\u306b\u3088\u308bPCR\u7523\u7269\u306b\u3088\u308b\u30de\u30fc\u30ab\u30fc\u30b7\u30b9\u30c6\u30e0\uff09\uff0c<\/p>\n

\"\"<\/p>\n

SSR\uff08\u5358\u7d14\u5869\u57fa\u53cd\u5fa9\u914d\u5217\uff09\u30de\u30fc\u30ab\u30fc<\/p>\n

 <\/p>\n

\"\"<\/p>\n

\u304c\u4f7f\u3048\u307e\u3059\uff0e<\/p>\n

\u3044\u307e\u306fPCR\u6cd5\u304c\u5229\u7528\u3067\u304d\u308b\u305f\u3081INDEL\u30de\u30fc\u30ab\u30fc\u306e\u958b\u767a\u3082\u3067\u304d\u307e\u3059\uff0e<\/p>\n

 <\/p>\n

\"\"\"\"<\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

\"\"<\/p>\n

PCR\u306b\u3088\u308b\u5897\u5e45\u5f8c\u306f\u96fb\u6c17\u6cf3\u52d5\u3092\u884c\u3063\u3066\u30d0\u30f3\u30c9\u306e\u8b58\u5225\u3092\u884c\u3048\u307e\u3059\uff0e<\/p>\n

\"\"\"\"<\/p>\n

 <\/p>\n

\u3053\u3061\u3089\u306f\u5728\u6765\u8d64\u7c73\u306e\u591a\u69d8\u6027\u3092\u8abf\u67fb\u3057\u305f\u3068\u304d\u306e\u3082\u306e\u3067\u3059\uff0e<\/p>\n

 <\/p>\n

\u5f53\u7814\u7a76\u5ba4\u3067\u306f\u9999\u308a\u7c73\u3082\u5229\u7528\u3059\u308b\u3053\u3068\u304c\u591a\u3044\u306e\u3067INDEL\u30de\u30fc\u30ab\u30fc\u3092\u5229\u7528\u3057\u3066\u5224\u5b9a\u3057\u3066\u3044\u307e\u3059\uff0e<\/p>\n

\"\"<\/p>\n

\u91ce\u751f\u30a4\u30cd\u96c6\u56e3\u89e3\u6790\u3067\u306fCAPS\u30de\u30fc\u30ab\u30fc\u958b\u767a\u3082\u884c\u3044\u307e\u3057\u305f\uff0e<\/p>\n

 <\/p>\n

 <\/p>\n

\"\"<\/p>\n

\u8abf\u67fb\u96c6\u56e3\uff08Google)\"\"<\/p>\n

\u8abf\u67fb\u5730\u306e\u73fe\u5834\u5199\u771f\"\"<\/p>\n

\u8fd1\u3065\u304f\u306b\u306f\u30ab\u30cc\u30fc\u3092\u5229\u7528\u3057\u307e\u3057\u305f\uff0e\u305d\u306e\u7d50\u679c\uff0c<\/p>\n

 <\/p>\n

\"\"<\/p>\n

\u8449\u7dd1\u4f53\u306e\u6bcd\u7cfb\u60c5\u5831\u3092\u5f97\u308b\u305f\u3081\u306b\u30b2\u30ce\u30e0\u60c5\u5831\u304b\u3089\u5236\u9650\u9175\u7d20\u3067\u5207\u65ad\u3067\u304d\u308b\u5834\u6240\u3092\u9078\u5b9a\u3057\u3066\uff0cPCR\u5897\u5e45\u5f8c\u306b\u6d88\u5316\u3057\u3066\u96fb\u6c17\u6cf3\u52d5\u3092\u884c\u3044\u307e\u3057\u305f\uff0e<\/p>\n

 <\/p>\n

 <\/p>\n

\"\"<\/p>\n

 <\/p>\n

\"\"<\/p>\n

 <\/p>\n

\u751f\u614b\u7279\u6027\u3092\u542b\u3081\u30661\u3064\u306e\u6c60\u306e\u5468\u8fba\u306e\u96c6\u56e3\u306e\u591a\u69d8\u6027\u304c\u6d6e\u304b\u3073\u4e0a\u304c\u3063\u3066\u304d\u307e\u3057\u305f\uff0e<\/p>\n

 <\/p>\n

 <\/p>\n

\uff0aPCR\u306b\u3088\u308b\u30de\u30fc\u30ab\u30fc\uff1aRAPD,SSR\uff0cCAPS\u306a\u3069\uff0e<\/span>\u5229\u70b9\uff1a\u5c11\u91cf\u306e\u8a66\u6599\u3067\u3088\u3044\uff0c<\/span>DNA\u591a\u578b\u691c\u51fa\u983b\u5ea6\u3082\u9ad8\u3044\u3053\u3068\u3067\u3059\uff0e<\/span><\/p>\n

RAPD\uff1a10mer\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3067\u6570\u591a\u304f\u306e\u30b2\u30ce\u30e0\u4e0a\u3067\u691c\u51fa\u3055\u308c\u308b\u90e8\u5206\u3092\u540c\u6642\u306b\u691c\u51fa<\/span><\/p>\n

SSR\uff1a\u30b2\u30ce\u30e0\u914d\u5217\u306e\u5fc5\u8981\u6027\uff0e\u5171\u512a\u6027\uff0e\u8fd1\u7e01\u54c1\u7a2e\u9593\u3067\u3082\u591a\u578b\u671f\u5f85\u3067\u304d\u308b\uff0e\u67d3\u8272\u4f53\u306e\u3042\u3089\u3086\u308b\u3068\u3053\u308d\u306b\u6563\u5728\uff0e\u3055\u3089\u306b\u5bc6\u306a\u30de\u30fc\u30ab\u30fc\u306fSNP\uff0e<\/span>http:\/\/archive.gramene.org\/markers\/microsat\/<\/span><\/p>\n

SNP:\u4e00\u5869\u57fa\u591a\u578b\uff0ePCR\u306b\u3088\u308a\u7279\u6b8a\u306a\u30bf\u30a4\u30d4\u30f3\u30b0\u306b\u3088\u308a\u591a\u578b\u3092\u307f\u308b\u3053\u3068\u304c\u53ef\u80fd\uff0e\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u60c5\u5831\u304b\u3089\u4f4d\u7f6e\u60c5\u5831\u3092\u660e\u3089\u304b\u306b\u3057\u3066\u304b\u3089\u7528\u3044\u308b\uff0e<\/span><\/p>\n

CAPS\uff1aPCR\u7523\u7269\u5185\u306e\u5236\u9650\u9175\u7d20\u591a\u578b\u3092\u691c\u51fa\u3059\u308b\u00a0<\/span><\/p>\n

\u30a4\u30cd\u5728\u6765\u7a2e\u306e\u5834\u5408\u306f\uff0c\u8fb2\u6c34\u7701\u306e\u30b3\u30a2\u30b3\u30ec\u30af\u30b7\u30e7\u30f3\u306b<\/a>\u304a\u3051\u308bSSR\u591a\u578b\u60c5\u5831\u306e\u958b\u793a\u3082\u306a\u3055\u308c\u3066\u3044\u307e\u3059\uff0e<\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

DNA\u306e\u591a\u69d8\u6027\uff1a \u591a\u578b\uff08\u305f\u3051\u3044\uff09\u3068\u3082\u3044\u3046DNA\u306e\u9055\u3044\u304c\u307f\u3089\u308c\u308b\u4f55\u304c\u308f\u304b\u308b\u306e\u3060\u308d\u3046\u304b\uff0e\u30a4\u30cd\u306e\u5728\u6765\u7a2e\u306e\u9055\u3044\uff0c\u54c1\u7a2e\u8b58\u5225\uff0cDNA\u306a\u3089\u30b3\u30e1\u3082\u500b\u4f53\u306e\u8b58\u5225\u304c\u3067\u304d\u307e\u3059\uff0e\u305d\u306e\u305f\u3081\u306bDNA\u62bd\u51fa\u3068\u591a\u578b\u306e\u6bd4\u8f03\u304c\u5fc5\u8981\u3067\u3042\u308a\uff0c\u305d\u306e\u65b9\u6cd5\u306b\u306f\u751f\u7269\u7a2e\u306b\u3088 […]<\/p>\n","protected":false},"author":216,"featured_media":0,"parent":0,"menu_order":150,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-577","page","type-page","status-publish","hentry","no-featured-image"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/home.hirosaki-u.ac.jp\/crobrdlab\/wp-json\/wp\/v2\/pages\/577","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/home.hirosaki-u.ac.jp\/crobrdlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/home.hirosaki-u.ac.jp\/crobrdlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/home.hirosaki-u.ac.jp\/crobrdlab\/wp-json\/wp\/v2\/users\/216"}],"replies":[{"embeddable":true,"href":"https:\/\/home.hirosaki-u.ac.jp\/crobrdlab\/wp-json\/wp\/v2\/comments?post=577"}],"version-history":[{"count":1,"href":"https:\/\/home.hirosaki-u.ac.jp\/crobrdlab\/wp-json\/wp\/v2\/pages\/577\/revisions"}],"predecessor-version":[{"id":592,"href":"https:\/\/home.hirosaki-u.ac.jp\/crobrdlab\/wp-json\/wp\/v2\/pages\/577\/revisions\/592"}],"wp:attachment":[{"href":"https:\/\/home.hirosaki-u.ac.jp\/crobrdlab\/wp-json\/wp\/v2\/media?parent=577"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}